cu zn superoxide dismutase  (Proteintech)

Journal: International Journal of Molecular Sciences

Article Title: Green Synthesized Titanium Oxide Nanoparticles Promote Salt Tolerance in Soybean

doi: 10.3390/ijms26178309

Figure Lengend Snippet: Immunoblot analysis of proteins involved in soybean treated with TiO 2 NPs under salt stress. Six treatments were performed: control, CS-TiO 2 NPs, GS-TiO 2 NPs, salt, CS-TiO 2 NPs + salt, and GS-TiO 2 NPs + salt. Proteins extracted from root and hypocotyl of soybean were separated on SDS-polyacrylamide gel by electrophoresis. Proteins were transferred onto PVDF membranes. The membranes were cross-reacted with anti-superoxide dismutase, peroxiredoxin, ascorbate peroxidase, and glutathione reductase antibodies. The integrated densities of the bands were calculated using ImageJ software. Data analysis and statistical calculations are the same as in (**, p < 0.01; *, p < 0.05).

Article Snippet: After blocking, the PVDF membrane was cross-reacted with the primary antibodies for 30 min. As the primary antibodies, anti-V ATPase (Agrisera, Vännäs, Sweden), ascorbate peroxidases [ ], glutathione reductase (Agrisera), Cu/Zn superoxide dismutase (Proteintech, Rosemont, IL, USA), and peroxiredoxin [ ] antibodies were used.

Techniques: Western Blot, Control, Polyacrylamide Gel Electrophoresis, Software

Journal: Experimental Physiology

Article Title: Effects of soluble guanylyl cyclase stimulation on muscle oxygenation and exercise capacity in heart failure with mildly reduced ejection fraction

doi: 10.1113/EP092756

Figure Lengend Snippet: Spinotrapezius muscle sGCβ1 (a) and SOD1 (b) protein expression. Data are means ± SD. Twenty‐one samples of spinotrapezius muscle (Healthy, n = 4; Healthy + BAY41, n = 4; HFmrEF, n = 6; HFmrEF + BAY41, n = 7) were used to measure sGCβ1 and SOD1 expression via western immunoblotting. Representative western blot images are presented below each graph. (a) There was a significantly greater expression of sGCβ1 in the spinotrapezius muscle of HFmrEF + BAY41 rats (* p = 0.045). (b) No statistical difference was detected in spinotrapezius muscle SOD1 expression (Healthy, p = 0.932; HFmrEF, p = 0.897). Data were analysed via two‐way mixed‐effects ANOVA and Fisher's least significant difference test without correction for multiple comparisons. Abbreviations: a.u., arbitrary units; BAY41, BAY 41‐2272; HFmrEF, heart failure with mildly reduced ejection fraction; sGCβ1, soluble guanylyl cyclase beta 1 subunit; SOD1, superoxide dismutase 1.

Article Snippet: Protein quantification in medial costal diaphragm lysates was completed with Jess Simple Western (ProteinSimple) automated western blot analysis with primary antibodies for soluble guanylyl cyclase subunit beta 1 (sGCβ1; NBP1‐89784, Novus Biologicals, 1:10 dilution) and superoxide dismutase 1 (SOD1; NBP1‐90186, Novus Biologicals, 1:100 dilution).

Techniques: Expressing, Western Blot